Adding a New Dimension
to DNA Sequencing
Single Technologies’s unique 3-D Sequencing™ technology allows us to read DNA in volumes, which drastically increases the number of DNA sequences that can be read in parallel and naturally bridges Next Generation Sequencing with Spatial Biology
Why increase the DNA Sequencing Bandwidth?
To Sequence Everyone
Identification of rare disease
Whole-genome sequencing for rare diseases has the power to help doctors diagnose genetic diseases quickly, helping families avoid long diagnostic odysseys. Roughly 8% of the population is expected to have a rare disease. Around 80% of rare diseases are of genetic origin and, of those, 70% appear already in childhood.
To Sequence Everything
A probe in molecular biology is often a single-stranded sequence of DNA used to search for its complementary DNA or RNA sequence in a sample genome or an antibody used to identify the presence of a specific protein. All probes could use an attached barcode tag with nucleotides that could be detected with sequencing. There is a strong demand to detect many more probes in parallel in multiomics or in search of disease specific biomarkers.
To Sequence Everywhere
Cell mapping and the true architecture of organs
Spatial sequencing - sequencing in-situ in cells or tissues - brings both a detailed understanding of individual cells and of the distribution of different cell types in a tissue. To reach its full potential many cells have to be read simultaneously and organ tissue has to be understood in three dimensions. The international Human Cell Atlas project aims at creating comprehensive reference maps covering every cell type, their functions and relations, of all 37 trillion cells in a human.
The Power of Theta
The world’s first 3-D sequencer
Theta has been developed to make a difference, where we have pushed the sequencing power closer to what nature allows us to do. Theta is an image-based sequencer built for large scale production of DNA data when bandwidth matters. The machine can read DNA libraries in both solution and in cells.
3-D sequencing is not limited to fragment libraries but can also sequence many reads in single cells and tissue using the same platform. Our unique flowcell architecture allows multiple and unconventional sample types. Both the signal detection for NGS sequencing and the imaging of cells or tissue are done at the same time in three dimensions at the limit of optical resolution.
Next Generation Sequencing will after the introduction of Theta no more be limited to surfaces and channels but analyzes instead by volume rather than area, increasing the potential number of reads in a run many times.
Our vision is removing the bottleneck of generating sequencing data allowing scientists, empowered with AI, to solve complex biological problems.
Johan Strömqvist – Founder/CEO
Under the hood
What is 3-D Sequencing™?
Single’s system is the world’s fastest true confocal scanner, imaging large areas in three dimensions at the optical resolution limit with single fluorescence molecule sensitivity.
Single’s unique open fluidics exchange liquids and reagents in three dimensions, allowing rapid distribution of liquids over large volumes and efficiently using valuable reagents.
Single’s platform performs massively parallel reactions in three dimensions, enabling the detection of sequences at all sites in a volume independent of sample or molecule.